Question: Reliability of Microarray data analysis through "Altanalyze" tool.
gravatar for Muhammad Haseeb
3 months ago by
Ajou University, Suwon, South Korea
Muhammad Haseeb20 wrote:

Hey Everyone,

I hope you all are doing good. I am interested in analyzing microarray data of tumor gene expression. I want to identify the differentially expressed gene among wildtype VS Knockout. And to check their role, I used the AltAnalyze tool for normalization, heatmap, clustering and pathway study. Is anyone who familiar with this tool and its reliability or suggest me good and reliable tool license free. Can someone recommend me good article/material, How to dig out the meaningful information/hypothesis from microarray data results? I am a beginner in this field, I will be highly obliged for your valuable suggestions. I will be waiting for your answers. Thank you.

ADD COMMENTlink written 3 months ago by Muhammad Haseeb20

AltAnalyze will produce a set of files in the folder GO-Elite under the folders upregulated, downregulated and regulated with pathway and ontology enrichment analyses that indicate likely regulated biologically relevant terms. The pruned results file will indicate these results and associated genes. In the folder ExpressionOutput you find the file with the prefix DATASET to sort and filter for the most significantly differentially expressed genes. I recommend filtering based on the adjusted p-value column which outputs an FDR adjusted empirical Bayes moderated t-test similar to limma in R. Note there are extensive gene and pathway annotations in this file. These and nearly all other results (colored pathways, heatmaps, networks, QC, PCA plots) can be browsed from the AltAnalyze interactive Results viewer (grey icon). For futher assistance contact the AltAnalyze help desk of view the online tutorial videos in YouTube.

ADD REPLYlink written 3 months ago by Nathan Salomonis30

Dear Nathan,

Thank you very much for such a nice share. I will follow your suggestions and will contact if needed.

Thank you,


ADD REPLYlink written 3 months ago by Muhammad Haseeb20

I'm not familiar with the software you mentioned, but Bioconductor/R tools might be useful, see following links to get started:
Analysing Microarray Data In Bioconductor
Using Bioconductor for Microarray Analysis

ADD REPLYlink modified 3 months ago • written 3 months ago by WouterDeCoster16k

Thank you very much for providing the link.


ADD REPLYlink written 3 months ago by Muhammad Haseeb20
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