Question: What 2 indicates in 2*150 bp, 2*300 bp maximum read length from Illumina website http://www.illumina.com/systems/sequencing-platforms.html?
0
gravatar for saranpons3
2.3 years ago by
saranpons360
saranpons360 wrote:

Dear Bioinformaticians,

I was looking at various Illumina sequencing platforms here http://www.illumina.com/systems/sequencing-platforms.html.

What 2 indicates in 2x150 bp, 2x300 bp maximum read length from Illumina website http://www.illumina.com/systems/sequencing-platforms.html?

I would be grateful if anybody can answer this?

ADD COMMENTlink modified 2.3 years ago by WouterDeCoster39k • written 2.3 years ago by saranpons360

I edited your post because you use (probably not desired) markup, e.g. * will result in text in italics

ADD REPLYlink modified 2.3 years ago • written 2.3 years ago by WouterDeCoster39k
1
gravatar for WouterDeCoster
2.3 years ago by
Belgium
WouterDeCoster39k wrote:

That refers to the direction(s) of sequencing: paired end sequencing. Essentially, in 2x150bp you are sequencing once 150bp in one direction of the molecule, and once in the other direction.

If you have a molecule of 400bp you would sequence 150bp from each side, leaving a gap of 100bp in the middle.

enter image description here

ADD COMMENTlink modified 2.3 years ago • written 2.3 years ago by WouterDeCoster39k

Hello WouterDeCoster,

Thanks for editing my question and the answer. If I assemble forward and reverse end reads where each of them are of length 150 bp , then saying "assembling the reads of 150 bp is correct" or saying "assembling the reads of 300 bp is correct" ?

Also, I'm looking for the Denovo assemblers which can assemble reads of length 300 bp. Do you know any assemblers which can assemble reads of 300 bp?

ADD REPLYlink modified 2.3 years ago by WouterDeCoster39k • written 2.3 years ago by saranpons360

No, you don't have reads of 300bp then. You have paired end 150bp reads.

For a bunch of tools for assembly, have a look at omictools. I can't give you more accurate advice for this, perhaps someone else.

ADD REPLYlink written 2.3 years ago by WouterDeCoster39k

Hello WouterDeCoster, Thanks for answering my question. As you said I'll look at omictools website.

ADD REPLYlink written 2.3 years ago by saranpons360
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