Hi, I have installed ncbi-blast-2.5.0+ in my ubuntu 16.04 system and downloaded the nr database using
$ ls *.gz |xargs -n1 tar -xzvf
and now I have nr db- nr.00 to nr.59.tar.gz (each having the 12 different files .phd,.phi, .phr upto .psq) But, when I try to run blastx, using the following command, for any -outfmt (like 0,1 or 6),
$ blastx -query test.fasta -db nr -outfmt 0 -out test_blastx -evalue 1e-10 It gives the following error. Error: NCBI C++ Exception: T0 "/build/ncbi-blast+-bzPf_D/ncbi-blast+-2.2.31/c++/src/corelib/ncbiobj.cpp", line 926: Critical: ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL pointer.
I know there are similar posts her on biostar but none solved my issue or if there is an identical post, I might have missed so, kindly direct me to it? Blast+ is working fine as I have used it for analysis of my data, creating my own databases. I am facing problem with nr db only.