My question is about techniques for finding protein complexes (e.g. AP-MS, yeast two-hybrid), and it's in response to a recent answer. From that answer (emphasis mine):
... detection is biased towards stable complexes in AP-MS experiments but that may not be the case if you derive complexes from some other type of experiments like yeast two-hybrid. Note also that it is usually assumed that complexes found in AP-MS experiments are stable.
If AP-MS is usually assumed to find stable protein complexes, are there similar assumptions for other methods? e.g. Does yeast two-hybrid find more or less stable complexes? Also, is there any citable, systematic study that covers this?
edit: I'm specifically wondering about the techniques listed in CORUM e.g. "MI:0007". But any insight will be helpful.
