Best Tools for Counting Reads before DE Analysis
0
0
Entering edit mode
7.1 years ago
oma219 ▴ 40

Hello,

I'm planning on using DESeq2 for DE Analysis and I was wondering what program would be the best to produce the gene counts if I had previously used STAR for Alignment? Also, what other programs could I use for analysis that use the same gene count file because I hoping to compare my results between program? Any help would be appreciated! Thanks.

Omar

RNA-Seq sequencing • 1.9k views
ADD COMMENT
1
Entering edit mode

As Wouter mentioned your previous question already has implementable options outlined. You need to go through some literature and tutorials (this, this and this review).

ADD REPLY
0
Entering edit mode

Your previous thread When is HTSeq Count Script used? contains already quite some information on that.

ADD REPLY

Login before adding your answer.

Traffic: 3256 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6