Question: why remove adapter just from 3' of the reads
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gravatar for jonessara770
2.6 years ago by
jonessara770170
jonessara770170 wrote:

Hello,

I wonder why we just remove adapter from 3' end of reads and not both sides? For example when using Illumina TruSeqâ„¢ Small RNA, they use RA5 and RA3 adapter, so why we just trim 3'?

For small RNA, do you trim for quality from both sides of a read if both sides does not have good quality?

Thanks Sara

rna-seq • 1.0k views
ADD COMMENTlink written 2.6 years ago by jonessara770170
3
gravatar for genomax
2.6 years ago by
genomax74k
United States
genomax74k wrote:

Take a look at the figure at the bottom of this page: http://nextgen.mgh.harvard.edu/IlluminaChemistry.html Unless your inserts are shorter then the length of sequencing you should never see adapters except on the 3'end.

ADD COMMENTlink modified 2.6 years ago • written 2.6 years ago by genomax74k
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