I am applying wgcna on my data set. My data set contains 20 samples. Out of these 20 samples, 10 are of smokers and 10 are of nonsmokers. 10 samples for each smokers and non- smokers are further divided into 5 samples from cancer patients and 5 from normal. The data is obtained from rna-seq platform. Raw counts are normalized by using vst normalization and quantile normalization. WGCNA has resulted in 29 modules. Problem i am facing is in trait module relation ship heatmap. I want to get modules that are highly correlated with cancer, smokers and non smokers. These three variables are taken as traits in trait file. The problem is that no module is significantly correlated with any trait. P-values are very large like 0.2, 0.4 etc.
Please help me finding what am i doing wrong?? Why there is no significance module ? Is that some problem of data normalization ?
Thankyou in advance..