Hi, I got my RNA-Seq expression Data in FPKM, then I pick some interested genes to validate by qPCR. The expression table of FPKM and qPCR in like below:
There gene FPKM is 0 in sample B. How can I validate the expression the log2 ration of sampleA / SampleB.
I have tried to replace 0 to 0.0001, so that I can compare the log2 ration of RNA-Seq and qPCR and make a linear regression. But the
r of regression is different when I replace 0 to another value.
Is there any good way to handle these data. These genes are in a pathway of my interested, I want to validate these genes expression in qPCR.