Edit (to be a fit for the site):
When cloning an integral membrane protein (~150AA/~462bp), two AA changes were introduced at the N or C terminal of the sequence, assuming these changes are real and not due to sequencing errors, which computational methods exist to predict:
- the effect of the mutations on membrane integration
- the 3D-structure
- the function of the protein
(now sounds like a homework question;)
Hello everybody, I cloned my gene with gibson asembly method, but after cloning and sequencing I got two point mutation in my sequence ..that bot of them are at the end of my protein sequence, which Isoleucine exchanged to Methionine. I want to check this can be effect on my protein structure and function or not? how should I check it ?
I am looking forward to your kink response.