This is somewhat similar to some older Biostars posts, but most of those are quite old and I'm wondering if there has been some consensus since.
I have been doing a number of analyses looking at overlap between regions of interest, and I would now like to apply a statistical test to determine if the overlap between these regions is significant. I am thinking that I can compare to overlap with random size-matched regions to generate a contingency table like this:
Regions of interest Random regions Overlapping value 1 value 2 Non-overlapping value 3 value 4
Then run a chi-square test. Does this seem reasonable to everyone else? Or will I get dinged by reviewers?