Question: Converting TPM to FPKM
1
gravatar for alexahes
17 months ago by
alexahes10
alexahes10 wrote:

Hello.

I have an expression dataset in TPM values that I want to convert to FPKM. The problem is that I have no idea how to do it and it seems like my google-skills are not good enough to find an answer either.

Does anyone here have any experience with converting TPM values to FPKM?

Many thanks!

rna-seq tpm fpkm • 1.0k views
ADD COMMENTlink modified 17 months ago by karl.stamm3.3k • written 17 months ago by alexahes10
1

Why would you do this, rather than converting FPKM to TPM? There is no benefit to FPKM over TPM and the FPKM to TPM conversion is trivial.

ADD REPLYlink written 17 months ago by Rob2.8k
2
gravatar for karl.stamm
17 months ago by
karl.stamm3.3k
United States
karl.stamm3.3k wrote:

Any conversion will have some error to it. Theoretically, the math is a quick conversion, but realistically, both of your data sets are produced by different tools with different biases. The conversion is not going to be correct. Better to try to use both sorts of data, knowing they are simply different. Maybe log-normalization can get you into similar scales without explicitly worrying about units.

ADD COMMENTlink written 17 months ago by karl.stamm3.3k
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