Question: Converting TPM to FPKM
gravatar for alexahes
19 months ago by
alexahes10 wrote:


I have an expression dataset in TPM values that I want to convert to FPKM. The problem is that I have no idea how to do it and it seems like my google-skills are not good enough to find an answer either.

Does anyone here have any experience with converting TPM values to FPKM?

Many thanks!

rna-seq tpm fpkm • 1.1k views
ADD COMMENTlink modified 19 months ago by karl.stamm3.4k • written 19 months ago by alexahes10

Why would you do this, rather than converting FPKM to TPM? There is no benefit to FPKM over TPM and the FPKM to TPM conversion is trivial.

ADD REPLYlink written 19 months ago by Rob2.9k
gravatar for karl.stamm
19 months ago by
United States
karl.stamm3.4k wrote:

Any conversion will have some error to it. Theoretically, the math is a quick conversion, but realistically, both of your data sets are produced by different tools with different biases. The conversion is not going to be correct. Better to try to use both sorts of data, knowing they are simply different. Maybe log-normalization can get you into similar scales without explicitly worrying about units.

ADD COMMENTlink written 19 months ago by karl.stamm3.4k
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1773 users visited in the last hour