how to design primers for AC112229.1 pseudogene and no for his parental gene ?

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7.0 years ago

reimco2 • 0

Hi every one, I'm trying to design primers for this pseudogene. But these peseudogene are not available in nbci nucleotide it's take a part of a clone, and his parental gene is (ZNF532). when i toke the sequence of pesudogene and design primers. primers cover or amplified the parental gene and not pseudogene. How to design primers in this case. Hope to hear advices from you.. Thanks a lot..

gene • 1.9k views

ADD COMMENT • link 7.0 years ago by reimco2 • 0

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Primers for what? Real-time qPCR? Regular genomic DNA amplification?

ADD REPLY • link 7.0 years ago by h.mon 35k

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So sorry. it was a mistake.. thanks .. i need to desing primers for Real-time qPCR( RT-qPCR) and my target is quantify pseudogene expression...thank you for your point.

ADD REPLY • link 7.0 years ago by reimco2 • 0

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Please use ADD COMMENT/ADD REPLY when responding to existing posts to keep threads logically organized.

ADD REPLY • link 7.0 years ago by GenoMax 142k

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