Question: Blastn stopped finishing commands it previously finished
gravatar for Jacob
3.1 years ago by
Jacob10 wrote:

I was running blastn on the command line queries like this one

blastn -query Acan -db nr -out "blastOutput/AcanHomo sapiens_blast.txt" -evalue 0.00001 -outfmt 6 -entrez_query "Homo sapiens[Organism] AND refseq[filter]" -remote

Were working fine. After I tried to run some commands like

nohup ./

screen ./

./ ^Z bg disown %1

The query's no longer finished I also ran about 60 or so queries in a loop. Does blast have some sort of limit for people?


ADD COMMENTlink modified 3.1 years ago • written 3.1 years ago by Jacob10

Are you running more than one -remote instance at the same time? This may be the problem:

The BLAST+ remote service uses a shared resource (the computers at the NCBI), so only one BLAST+ application should run remote searches at a time.

ADD REPLYlink modified 3.1 years ago • written 3.1 years ago by h.mon30k
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