I am using makeblastdb to create a database of fungal ITS sequence. When I run the command:
./makeblastdb -in uniteits.fasta -out UNITE_ITS.fasta -dbtype nucl -parse_seqids
It appears to run, fine and I get the following output:
Building a new DB, current time: 05/15/2017 16:18:17 New DB name: /Users/user/Documents/BLAST/ncbi-blast-2.6.0+/bin/UNITE_ITS.fasta New DB title: uniteits.fasta Sequence type: Nucleotide Deleted existing Nucleotide BLAST database named /Users/user/Documents/BLAST/ncbi-blast-2.6.0+/bin/UNITE_ITS.fasta Keep MBits: T Maximum file size: 1000000000B Adding sequences from FASTA; added 29342 sequences in 1.83726 seconds.
However, when I search for the databases, it is not there. I double checked that it wasn't just somewhere else on my computer, but still nothing. I've read some previous posts suggesting that the issue may be something to do with permissions, but did not provide a solid answer. I tried using chmod, if this was in fact the case, but to no avail.
Can anyone help me please?