Question: Sequence Header Convertion from Nanopore to PacBio
gravatar for dani357
2.2 years ago by
dani3570 wrote:

I want to use the FALCON assembler. I did sequencing using Oxford naopore MinION. I need to convert the sequence header from nanopore to PacBio to assemble using FALCON. I appreciate any information. Thank you.

alignment assembly • 1.1k views
ADD COMMENTlink modified 11 months ago by Ric250 • written 2.2 years ago by dani3570

Did you have any luck adjusting minion reads for use with falcon? and/or falcon unzip?

ADD REPLYlink written 15 months ago by samuel.a.odonnell10

This request sounds unusual at best. FALCON diploid assembler is designed for PacBio data. You are not going to have subreads files etc. Would be interesting if anyone has tried something like this.

ADD REPLYlink modified 2.2 years ago • written 2.2 years ago by genomax70k

The assembler CANU would appear to be a better option.

ADD REPLYlink written 11 months ago by colindaven1.6k
gravatar for Ric
11 months ago by
Ric250 wrote:

DASCRUBBER-wrapper has a method called rename_reads_with_fake_pacbio_names. I think you convert the subreads to fasta file.

ADD COMMENTlink written 11 months ago by Ric250
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