blast error: Syntax error in system-wide configuration file: NCBI C++ Exception

Question

[lidanqing1990]

hi，everyone！ I use blast+ to make annotation of RNA-seq data against swissprot When I run blast, it always stopped by error. I used blast 2.3.0 and my commands is:

blastx -query /home/aubdyl/purple_nutsedge/purple_nutsedge_trinity/Trinity.fasta -out /home/aubdyl/purple_nutsedge/purple_nutsedge_trinity/purple_nutsedge_swissprot -db /home/aubdyl/data/blast/swissprot.00 -outfmt 6 -evalue 1e-5 -num_descriptions 10 -num_threads 4

and the error is:

Critical: (110.6) CNcbiRegistry: Syntax error in system-wide configuration file: NCBI C++ Exception:
    T0 "/home/coremake/release_build/build/PrepareRelease_Linux64-Centos_JSID_01_250088_130.14.22.10_9008__PrepareRelease_Linux64-Centos_1448906370/c++/compilers/unix/../../src/corelib/ncbireg.cpp", line 675: Error: ncbi::IRWRegistry::x_Read() - Invalid registry entry format: '$ cat .ncbirc  ' (m_Pos = 1)

Warning: [blastx] The parameter -num_descriptions is ignored for output formats > 4 . Use -max_target_seqs to control output
Error: NCBI C++ Exception:
    T0 "/home/coremake/release_build/build/PrepareRelease_Linux64-Centos_JSID_01_250088_130.14.22.10_9008__PrepareRelease_Linux64-Centos_1448906370/c++/compilers/unix/../../src/corelib/ncbireg.cpp", line 675: Error: ncbi::IRWRegistry::x_Read() - Invalid registry entry format: '$ cat .ncbirc  ' (m_Pos = 1)

I have changed another edition of blast, but it still doesn't work. Could anyone give me some help ? Thank you so much!

[GenoMax]

Did you edit the .ncbirc file manually and make any changes?

Also try changing -db /home/aubdyl/data/blast/swissprot.00 to -db /home/aubdyl/data/blast/swissprot.

[lidanqing1990]

Thanks, I didn't edit .ncbirc file manually and -db /home/aubdyl/data/blast/swissprot doesn't work. Could you tell me where could I find .ncbirc file ?

[GenoMax]

It should be in your home directory. See if you are able to cat ~/.ncbirc?

[lidanqing1990]

Yes, I saw it. Should I delete this file? Blast+ Error seems similar to my question.

[GenoMax]

Move the file to a new name e.g. mv ~/.ncbirc ~/.ncbirc.ORIG and try.

[lidanqing1990]

Many thanks, I deleted that file and commands are running well at present.