Question: Blast+ Error
0
gravatar for shiy05
6.5 years ago by
shiy05280
shiy05280 wrote:

hi,everyone! I use blast+ to make annotation of RNA-seq data against nr. When I run blast, it always stopped by error. my command line is "blastn -task blastn -query Unigene.fa -db human.rna.fna -out NGB_blast.txt" and the error is "Critical: (110.6) CNcbiRegistry: Syntax error in system-wide configuration file: NCBI C++ Exception: "..........\src\corelib\ncbireg.cpp", line 660: Error: ncbi::IRWRegistry ::x_Read() - Badly placed '\' in the registry value: 'ROOT=J:\nASNLOAD=J:\BioEdi t\tables\nDATA=J:\BioEdit\tables\' (m_Pos = 4)

Error: NCBI C++ Exception: "..........\src\corelib\ncbireg.cpp", line 660: Error: ncbi::IRWRegistry ::x_Read() - Badly placed '\' in the registry value: 'ROOT=J:\nASNLOAD=J:\BioEdi t\tables\nDATA=J:\BioEdit\tables\' (m_Pos = 4)" I thought something is wrong with my bioedit, so I uninstalled it. However, the error continue to shows up. what should I do?

blast • 5.2k views
ADD COMMENTlink modified 2.4 years ago by bhaltli10 • written 6.5 years ago by shiy05280
1

'ROOT=J:\nASNLOAD=J:\BioEdi t\tables\nDATA=J:\BioEdit\tables\'

Is there a space in "BioEdit" or is that from the copy/paste-process?

ADD REPLYlink written 6.5 years ago by Philipp Bayer5.8k

no, I didn't copy/paste but type by myself.

ADD REPLYlink written 6.5 years ago by shiy05280

I had the same problem, and can not find the ncbi.ini file under the bioedit/tables.

Any suggestions are appreciated.

ADD REPLYlink written 5.7 years ago by jlpeng750
1
gravatar for Leonor Palmeira
6.5 years ago by
Leonor Palmeira3.6k
Liège, Belgium
Leonor Palmeira3.6k wrote:

There is a similar problem here where one solution was to:

  • find and delete the "ncbi.ini" file from your Windows file system (because corrupted by BioEdit).

I would rather copy it somewhere safe and restore it to its place if this does not do the trick.

ADD COMMENTlink written 6.5 years ago by Leonor Palmeira3.6k
1

Agreed, and the file will be $HOME/.ncbirc, for linux~

ADD REPLYlink written 6.5 years ago by GAO Yang250

I can't find it. My OS is windows XP.

ADD REPLYlink written 6.5 years ago by shiy05280

win7 in c:\windows

ADD REPLYlink written 5.5 years ago by 1949mj0
1
gravatar for bhaltli
2.4 years ago by
bhaltli10
bhaltli10 wrote:

Hi

I had the same problem but trying to use MultiGeneBlast. I found the ncbi.ini in the following location on a laptop running Windows 7Pro: C:\Users\default.SID18883\AppData\Local\VirtualStore\Windows. Removing this file fixed the error.

ADD COMMENTlink written 2.4 years ago by bhaltli10
0
gravatar for heslopharrison
4.8 years ago by
heslopharrison0 wrote:

Similar thing happens with Geneious 7 on Windows 7 64bit. I had had BioEdit on the computer, then changed some file associations, and then the Geneious custom-blast gave an error similar to the above. Simply deleting two copies of NCBI.ini in Windows system directories (61 bytes, appearing to be unchanged for 3 years) let the Geneious blast work.

ADD COMMENTlink written 4.8 years ago by heslopharrison0
0
gravatar for balasink
3.7 years ago by
balasink10
Canada
balasink10 wrote:

Yea I have the same thing popping up. I uninstalled bioedit - no change, reinstalled everything -no change.

Using Windows 7 - it was working fine until something went corrupted. I can't even find the files that its saying is corrupted.

 

Please help =(

ADD COMMENTlink written 3.7 years ago by balasink10
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