Hello guys. I have generated a pool of random mutant bacteria using Tn4351 (a modified Tnseq tool) and am now trying to build a mutant library. Apparently I now need MmeI enzyme / adaptor / ligase / primers ... Are all these stuffs available online, or should I design my own adaptor / primers based on the specific vector that was used?
Also, are there any platform that could do all these things for me as long as I send them my DNA samples?
Thanks for your time!