Tn Seq protocol
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3.8 years ago

Hello guys. I have generated a pool of random mutant bacteria using Tn4351 (a modified Tnseq tool) and am now trying to build a mutant library. Apparently I now need MmeI enzyme / adaptor / ligase / primers ... Are all these stuffs available online, or should I design my own adaptor / primers based on the specific vector that was used?

Also, are there any platform that could do all these things for me as long as I send them my DNA samples?

Thanks for your time!

Tn-seq • 1.6k views
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This sounds more like a technical - sequencing question than bioinformatics, no?

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