Hi,
I have RNA-Seq data from virus infected cell. I would like to get the counts for both viral and host transcripts. I added the virus fasta sequence to the human genome as an additional "chromosome" so that I could align to both the viral and human DNA at the same time. But I don't know how to combine the GTF files to get the counts for both. I used htSeq count independently with the viral and human GTF files to get the counts for each separately. But I was wondering if there is a way to use a joint GTF file. I would appreciate any help!
Thanks. It worked for me.
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