**0**wrote:

Hello everyone,

I have the allele frequencies at many loci of 26 populations. I was wondering if anybody knows how to calculate the Fst for each variation. I am trying to find variations that are characteristics of a certain population/ethnicity (by selecting those variations with Fst < 0.05).

So for example, if I have 5 populations (A, B, C, D, E) and the allele frequencies at one locus (see below), how can I find the Fst for each variation at that position?

```
A->G A->T A->AC
A 0 0.94 0.05
B 0.07 0.15 0.1
C 0.8 0.1 0.04
D 0.1 0.05 0.2
E 0.03 0.1 0.15
------------------------------
Fst ?? ?? ??
```

I hope my understanding of Fst is correct. If not, please correct me.

Thanks