Entering edit mode
6.8 years ago
Dipro
•
0
Hi,
I was wondering if I could get some advice on controlling for random effects using DESeq2. For example, given simulated data:
Treatment Batch Estrus
Control 1 1
Control 2 2
Control 3 1
Control 4 1
Control 1 2
Control 2 2
Control 3 2
Control 4 1
Control 1 3
Control 2 1
Treated 3 3
Treated 4 3
Treated 1 2
Treated 2 3
Treated 3 2
Treated 4 3
Treated 1 2
Treated 2 1
Treated 3 1
Treated 4 2
What is the best way to control for batch and estrus? I understand that DESeq2 does not support random effect analysis. As such, I was wondering if I could be advised on a work around for this type of analysis?
Thanks
Hello Dipro!
It appears that your post has been cross-posted to another site: https://support.bioconductor.org/p/98425/ (and answered there)
This is typically not recommended as it runs the risk of annoying people in both communities.