Question

convert SRA file into forward and reverse fastq files

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6.8 years ago

sunna_j • 0

I have downloaded .sra data from the SRA (Study SRP022224, Run SRR848976) with SRA Toolkit. I tried to convert it into the fastq files with fastq-dump, but I am not getting two files (one forward, one reverse). I'm just getting one fastq-file. What is my fault?

next-gen sequencing • 4.0k views

ADD COMMENT • link updated 5.6 years ago by gtrwst9 • 0 • written 6.8 years ago by sunna_j • 0

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Get the fastq files directly from EBI-ENA.

ADD REPLY • link 6.8 years ago by GenoMax 141k

score 1 · Answer 1 · 2017-07-24

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6.8 years ago

EagleEye 7.5k

A: Downloading data from Short Read Archive

ADD COMMENT • link 6.8 years ago by EagleEye 7.5k

score 0 · Answer 2 · 2018-09-07

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5.6 years ago

gtrwst9 • 0

fastq-dump --split-3 SRR...

This does NOT redownload everything.

ADD COMMENT • link 5.6 years ago by gtrwst9 • 0