I am new in DEA and I have RNA-Seq data already normalised to TPM, I calculated the log2FC values but I would like to calculate the p-values, FDR or t-values. Is there a way to do this kind of analysis in R? Here people discuss about it but the data is normalised in a different way and they didn't really answer the question. Any help is very appreciated.
Hi Spacebio,
Do not make redundant posts with similar question. I found this thread started by you TPM to logFC and pvalues
Hi EagleEye, I'm sorry! I am learning to use this platform to ask questions or debate about some topics. I couldn't find my old question, for that reason I started a new one. Please be a bit patient, slowly but surely I am learning to handle the info here.