Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?

Question

[WUSCHEL]

If a same gene is clone either endogenous promoter or constitutive promoter (35S), Do we get a significantly different protein abundance?

[Jean-Karim Heriche]

This is not a bioinformatics question and would be more appropriate to biology.stackexchange.

Answer 1

[Jean-Karim Heriche]

This outcome is unpredictable as far as I know. This would depend on the relative "strength" of the two promoters, whether and how they would be regulated in the cell you're using. The type of expression/overexpression you're using is also a factor, e.g. if doing stable transfection with random integration in the genome, your clone will be subject to regulation by the chromatin environment it ends up in.