Question: Minimum numbers of sequence bases in Sanger sequencing
0
gravatar for a.alsayed.phd
11 weeks ago by
a.alsayed.phd0 wrote:

If I am working on Sanger sequencing results for a certain type of viruses, by assays that target parts of the genome, so I am using few assays for each sample, got a couple of sequencing reads for each sample. Then, assembly, MSA, then phylogenetics: Please, is there any recommended minimum sequence length (seq, bases number) for each read to consider it successful?, for example if after trimming of the low quality ends, I got read with 35 bp length?? can I consider it a successful sequencing? and can I use that read in MSA etc? of note, the other reads are of hundreds length.

ADD COMMENTlink modified 8 weeks ago by h.mon9.2k • written 11 weeks ago by a.alsayed.phd0
1
gravatar for h.mon
8 weeks ago by
h.mon9.2k
Brazil
h.mon9.2k wrote:

Typical good Sanger reads will have between 600-900bp. Of course, sequencing length shouldn't be longer than whatever template you are sequencing. There are some features of the template that can interfere with sequencing, making reads shorter than they should - for example, long poly-A or poly-T (probably poly-C and poly-G as well), or if your template has length polymorphism.

ADD COMMENTlink written 8 weeks ago by h.mon9.2k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1342 users visited in the last hour