Question: Any suggestion for sanger sequencing result editing
gravatar for liaoyunshi
2.7 years ago by
liaoyunshi0 wrote:

Hi everyone,

I just do bi-directional sanger sequencing of my PCR products. I wonder if there is any free software can do the following things 1. check the chromatogram of sequencing results 2. edit the basecalling reads manually 3. automatically align forward and reverse reads, and do the 1. and 2. in one operation window.


chromatogram sequencing sanger • 1.3k views
ADD COMMENTlink modified 2.7 years ago by trausch1.5k • written 2.7 years ago by liaoyunshi0
gravatar for h.mon
2.7 years ago by
h.mon29k wrote:

Staden can, but there is a learning curve.

ADD COMMENTlink written 2.7 years ago by h.mon29k
gravatar for trausch
2.7 years ago by
trausch1.5k wrote:

We recently set up the web application Indigo that can analyze Sanger traces and aligns them to a reference genome or wildtype chromatogram. The standalone command-line version has a couple more features like dumping the whole Sanger trace as a tab delimited file so you can then indeed edit basecalls manually.If there is some feature missing just open an issue in the github repository of Indigo.

ADD COMMENTlink modified 5 months ago • written 2.7 years ago by trausch1.5k
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