Question: Any suggestion for sanger sequencing result editing
0
gravatar for liaoyunshi
21 months ago by
liaoyunshi0
liaoyunshi0 wrote:

Hi everyone,

I just do bi-directional sanger sequencing of my PCR products. I wonder if there is any free software can do the following things 1. check the chromatogram of sequencing results 2. edit the basecalling reads manually 3. automatically align forward and reverse reads, and do the 1. and 2. in one operation window.

Thanks.

ADD COMMENTlink modified 21 months ago by trausch1.3k • written 21 months ago by liaoyunshi0
0
gravatar for h.mon
21 months ago by
h.mon26k
Brazil
h.mon26k wrote:

Staden can, but there is a learning curve.

ADD COMMENTlink written 21 months ago by h.mon26k
0
gravatar for trausch
21 months ago by
trausch1.3k
Germany
trausch1.3k wrote:

We recently set up the web application Indigo that can analyze Sanger traces and aligns them to a reference genome or wildtype chromatogram. The standalone command-line version has a couple more features like dumping the whole Sanger trace as a tab delimited file so you can then indeed edit basecalls manually.If there is some feature missing just open an issue in the github repository of Indigo.

ADD COMMENTlink written 21 months ago by trausch1.3k
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