I am going to do genome assembly for that i did kmer analysis to see that which kmer value is good for the assembly. genome is heterozygous so i found two peaks in kmer analysis. i performed kmer analysis using kmer value 21, 32 and 64 now i am confused which kmer value is best for assembly. At kmer value=64, i got large genome size. Please explain which parameter i will see for good assembly.
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Thanks in advance