I have Denovo Assembly sequence from PacBio sequence.
It is around 2.9 GB size of sequences.
I'd like to know what is best alignment tool to analyze this data.
I am usually using this "blasr" to align HG19 to Sub reads. However, When I align HG19 to Denovo assembly results, It throwing the error. It looks like too large sequence to align it.
Could anyone know what is best practice to do it? I want to see and have bam file to visualize in IGV program.
Thank you in advance.