I am confused about normalization and statistics behind DE programs, I am using edgeR to analize two condittions.
Example for a gene ( raw-counts) four replicates by condition. Control (C) and treatment (T) of a gene:
Controles = 820-1618-1728-1007
Result of edgeR
logFC =1.10 logCPM = 6.5 LR = 9.77 PValue = 0.0017 FDR= 0.02
Why FBgn0034710 gene is statistically significant if one replicate has a lot of raw counts (7915) in comparation with the others. I know that library size could be a factor but this is similar in the other replicates