Question: How reads are divided into taxonomy level in Qiime?
gravatar for agata88
3 months ago by
agata88630 wrote:

Hi all!

I was testing and scripts for my own reference from NCBI.

My reference was created as GreenGenes reference (fasta and taxonomy file), except that mine contains only species taxonomy (full 16S gene). Since I've made it compatible with qiime I observed that after script all reads are somehow divided into all 7 levels....

Can you explain how works? Is there some kind of thresholds (%?) for phylum, class, order, family, genus, species level that helps calculate number of assigned reads?

Any help will be much appreciated :)

Best, Agata

qiime 16s • 226 views
ADD COMMENTlink modified 3 months ago by kennethcondon20071.1k • written 3 months ago by agata88630

This may help:

ADD REPLYlink written 3 months ago by kennethcondon20071.1k
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