Question: warning message after HTSeq
3 months ago by
BioDH • 0
BioDH • 0 wrote:
I have analyzed RNA-seq data with HTSeq.
My command that I used is
python -m HTSeq.scripts.count -f bam -r pos -s reverse -t ORF -i group -m union input.bam my.gff > output.txt
Warning message is
Warning: Mate records missing for 3752 records; first such record: <SAM_Alignment object: Paired-end read 'K00198:150:HGYHGBBXX:6:2219:20435:16049' aligned to chrII:[2341669,2341744)/+>. 11597356 SAM alignment pairs processed.
Do I have to concern the message? What does it mean?
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