Insert size is so small, good or bad?
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6.6 years ago
Tania ▴ 180

If you have the average insert size is ~158 estimated using BBMap . While the read length from the fastq file is 150. so we paired reads length (2*150)

This means the paired end reads overlap by too much. Is this good or bad?

Insert-size RNA-Seq • 4.0k views
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Bad, the insert size of my some bam files is one or zero. Then, I use the manta to call SV, it failed.

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6.6 years ago
Sharon ▴ 610

This is a related good read https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3906532/

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