Lnc RNA,RNA Seq

Question

[sa1987]

hi

I have a RNA seq data and planning to do LNC rna analysis.

LNC Rna analysis can be done only on small RNA seq or can be done using Rna seq data?

Answer 1

[Devon Ryan]

The l in lncRNAs stands for long, which in this case is >200 bases. Given that, an sRNAseq library prep would get rid of most lncRNAs. You need a ribo-depleted RNAseq dataset (the typical prep uses polyA selection, which will exclude some non-coding RNAs).

[sa1987]

hi

thank you for helping me out.i am working on already available RNA seq data from SRA. So how do i find its a ribo-depleted RNA seq.

[Devon Ryan]

You'd have to look at whatever metadata is provided, usually this is mentioned somewhere in there.

Answer 2

[Venkatesh]

This tutorial will give you the analysis workflow for LNC-RNA

Download the tutorial..

http://short.up-load.one/d/CXIY