**250**wrote:

I am trying calculate Z score of expression from RANseq dataset Ultimate goal is to generate similar figure as in Supplementary Fig 2 B and C of https://www.nature.com/articles/nature24489 I read a discussion (calculate z-score from rpkm values.) that Z score may not be useful in showing the expression changes. Are there any additional steps after simple calculation of z score that may be needed to generate a matrix close to above Fi2 and C above? Any thoughts or suggestions what should be best way to represent change in gene expression using Z score

**61k**• written 2.6 years ago by rob.costa1234 •

**250**