Question: Z score in RNAseq
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gravatar for rob.costa1234
5 weeks ago by
rob.costa1234140
United States
rob.costa1234140 wrote:

I am trying calculate Z score of expression from RANseq dataset Ultimate goal is to generate similar figure as in Supplementary Fig 2 B and C of https://www.nature.com/articles/nature24489 I read a discussion (calculate z-score from rpkm values.) that Z score may not be useful in showing the expression changes. Are there any additional steps after simple calculation of z score that may be needed to generate a matrix close to above Fi2 and C above? Any thoughts or suggestions what should be best way to represent change in gene expression using Z score

zscore rna-seq • 272 views
ADD COMMENTlink modified 5 weeks ago by Kevin Blighe11k • written 5 weeks ago by rob.costa1234140
2
gravatar for Kevin Blighe
5 weeks ago by
Kevin Blighe11k
London/Brazil
Kevin Blighe11k wrote:

Gene expression values are generally converted to Z-scores for visualisation purposes, like, in heatmaps. The differential expression analysis itself is performed on normalised counts, prior to log and/or Z-score transformation. Any data can be converted to Z-scores, including FPKM values. The point again, however, is that the Z-score transformation is purely for visualisation.

I don't see any major steps that need to be further applied after Z-score transformation for Figure 2B. Figure 2C, however, looks like the plotting of the first 3 dimensions after k-means clustering (hope that we're looking at the same figures).

Kevin

ADD COMMENTlink written 5 weeks ago by Kevin Blighe11k
1

Great! I was missing K means point.

ADD REPLYlink written 5 weeks ago by rob.costa1234140
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