Question: Multiplexed Rnaseq
5
gravatar for Yannick Wurm
7.9 years ago by
Yannick Wurm2.3k
Queen Mary University London
Yannick Wurm2.3k wrote:

So now that Illumina HiSeq can produce a gazillion bp of sequence per lane, what's the deal with multiplexing RNA samples? Say with this kit.

Has anyone had experience with data from a multiplexed RNAseq run? Do the data look ok? Is the cost of preparing 12 tagged libraries = 12x the cost of preparing one normal library?

gene illumina rna • 2.3k views
ADD COMMENTlink written 7.9 years ago by Yannick Wurm2.3k
3
gravatar for Istvan Albert
7.8 years ago by
Istvan Albert ♦♦ 77k
University Park, USA
Istvan Albert ♦♦ 77k wrote:

I have asked our lab manager about this (we have experience with ChiP-Seq if that matters at all), she tells me that:

The cost of preparing 12 tagged libraries (by which I am assuming you mean 12 tagged samples pooled into 1 library) is the same as preparing 1 single library.

ADD COMMENTlink written 7.8 years ago by Istvan Albert ♦♦ 77k

Thanks Istvan. Our core facility manager seems to differ: He says the cost of preparing a library of 12 tagged samples is closer to the cost of preparing 12 libraries than to the cost of preparing one single library...

ADD REPLYlink written 7.8 years ago by Yannick Wurm2.3k

Interesting - I wonder if the discrepancy is due to perhaps accounting for the actual sequencing cost as well (in our case at least).

ADD REPLYlink written 7.8 years ago by Istvan Albert ♦♦ 77k
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