So now that Illumina HiSeq can produce a gazillion bp of sequence per lane, what's the deal with multiplexing RNA samples?
Say with this kit.
Has anyone had experience with data from a multiplexed RNAseq run? Do the data look ok? Is the cost of preparing 12 tagged libraries = 12x the cost of preparing one normal library?
I have asked our lab manager about this (we have experience with ChiP-Seq if that matters at all), she tells me that:
The cost of preparing 12 tagged
libraries (by which I am assuming you
mean 12 tagged samples pooled into 1
library) is the same as preparing 1
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