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• Paired end reads merged to single end for mapping - should i allow multi-overlap similar to paired end reads?
  I am using featurecounts after mapping of my reads. I have a paired-end sequencing data but Only ...
• Is It Ok To Use One End Of A Set Of Paired-End Reads As A Set Of Single Reads?
  Hi, I'm wondering if it's OK to use one end of a set of paired-end reads as a set of single read...
• 16S reads comparing
  Hi, community, I have a question on the sequencing data comparison. My reads are PAIR ENDED RE...
• converting paired end reads to single end, is this a good idea?
  Hi dear one, I have paired end data and single end data (both are different single cell data), I...
• converting RF strand-specific mRNA sequencing data to FR stand-specific
  Dear all, We have carried out stand-specific mRNA sequencing (using the Illumina paired end pro...
• Raw reads from Ion Torrent. Is it single-end reads or paired-end reads.
  Hi guys, I got a raw data generated from Ion Torrent (Ion PGM) for sequencing virus species. The...
• Distance to reads ends
  Hello everyone! Im using SAMTOOLS, GATK UG and HC for SNP calling from target sequencing paired-e...
• Why Samtools cannot call all the SNPs from bam files?
  Dear all, My data is RNAseq with single end reads. I used the Bowtie to generate the Bam file. A...
• Single end reads to look like paired end on Galaxy
  Is there anyway to make single end reads look like a couple of paired end reads(split into two fi...
• Question about the type of SRA data (SRR9713131, single- or pair-end?)
  Hi, I am planning to re-analyze the scRNA-seq data on SRA (SRR9713131, https://www.ncbi.nlm.nih....
• Are single-end reads processed the same as paired-end reads regarding strandedness?
  I have RNA-seq data made from Illumina TruSeq Stranded mRNA Sample Preparation Kit. Some of my sa...
• differential expression analysis-- paired-end and single-end
  Is it possible to compare gene expressions of two types of sequencing data, i.e. treated samples ...
• Trimming single end reads for STAR?
  Hi, I just started to work with single end reads, which are already trimmed for adapter sequence...
• Why is my fastq data on two files if I am supposed to have single end reads?
  Weirdly enough, my single end reads are on two separate fastq files. Do you know why this happens...
• How does CIRCexplorer2 work with paired-end data?
  Hi everyone! I have been using CIRCexplorer2 in single-end RNA sequencing datasets for a while, ...
• 16S Sanger single end read assembly
  Hi, I have a single-end 16S Sanger sequencing generated using two primers (27F and 785F). I want...
• Average Snp Position For Reads With Predicted Snp
  Hi, I have a human sequencing data, and reads have been mapped with bwa. Any suggestions on what...
• How to deal with single reads from PE reads after trimming?
  I have some single reads from paired-ended reads data after trimming the reads. Should I map thes...
• CCCCCTTTTTTTTTTT reads in RNA-seq
  I have had some RNA-seq data sets (both single-end and paired-end) with (C)n(T)n sequences. E.g....
• EstimateLibraryComplexity alternative for single-end sequencing?
  Hi, I have an RNAseq dataset which was sequenced out with single-end settings. I would like to c...
• De novo assembly around 1 short length (<100bp) known sequence.
  Hello all, In an effort to avoid RACE-pcr my group is trying to use some sequencing data we have...