Hi, I was trying to build the txdb file using the makeTxDbFromBiomart, however, I came across with an error.
The command I typed was :
dicty_txdb <- makeTxDbFromBiomart(biomart="protists_mart", + dataset="ddiscoideum_eg_gene", + host="protists.ensembl.org")
The error message shows:
Download and preprocess the 'transcripts' data frame ... Error in getBM(attributes, filters = bm_filters, values = bm_values, ...) : Query ERROR: caught BioMart::Exception::Database: Could not connect to mysql database protists_mart_38: DBI connect('database=protists_mart_38;host=hh-mysql-eg-mart-web.ebi.ac.uk;port=4599','ensro',...) failed: Can't connect to MySQL server on 'hh-mysql-eg-mart-web.ebi.ac.uk' (111) at /nfs/public/release/ensweb/live/eg_mart/www_91/protists/lib/BioMart/Configuration/DBLocation.pm line 98.
Could you give me some suggestions?