Genomics, Proteomics, Transcriptomics, bacteria

Question

[abdulsaboor131990]

Hello I have proteomic data of bacteria in FASTA form taken at stationary and exponential growth phase of bacteria in the presence and absence of end product. I want to identify key proteins that are responsible for tolerance to the end product of this bacteria. My questions are 1) how can I compare these fasta files (any tool or method) to identify the expressed proteins at exponential growth phase in the presence and absence of end product? 2) How can I identify the potential protein that are responsible for tolerance to the end products?

[WouterDeCoster]

I have proteomic data of bacteria in FASTA form

Can you elaborate how you got this data?
I'm not sure of which assay fasta is the output.

And please select a more meaningful title for your question(s) and select appropriate tags.

[Kevin Blighe]

For your transcriptomic FASTA data, do the following:

1. Use Rockhopper to de novo assemble the transcriptomes and compare one condition versus the other
2. Use blastx to infer functionality of the differentially expressed transcripts from point 1

[GenoMax]

Even though the title has everything in it, the only real data mentioned in the post is proteomic. It may be in the form of short peptides in fasta format.

[WouterDeCoster]

Yes, but could be interesting to know if it's a quantitative assay to do differential expression analysis.