**460**wrote:

Hello All
**How to you think about this relatedness results?**

When I use relatedness2 in vcftools I got this:

```
vcftools --gzvcf p123.vcf.gz --relatedness2
INDV1 INDV2 N_AaAa N_AAaa N1_Aa N2_Aa RELATEDNESS_PHI
p1 p1 47388 0 47388 47388 0.5
p1 p2 28084 0 47388 39778 0.32219
p1 p3 27242 0 47388 47505 0.287081
p2 p1 28084 0 39778 47388 0.32219
p2 p2 39778 0 39778 39778 0.5
p2 p3 28753 0 39778 47505 0.329423
p3 p1 27242 0 47505 47388 0.287081
p3 p2 28753 0 47505 39778 0.329423
p3 p3 47505 0 47505 47505 0.5
```

But when I use that relatedness in vcftools I got this:

```
vcftools --gzvcf p123.vcf.gz --relatedness
INDV1 INDV2 RELATEDNESS_AJK
p1 p1 0.428714
p1 p2 -0.187837
p1 p3 -0.343416
p2 p2 0.552425
p2 p3 -0.171243
p3 p3 0.423852
```

**P2 is supposed to be the son of p3(father) and p1(mother) or this is what We wanna prove. It was not normal birth, a kind of artificial insemination**

I am surprised that the PHI score between P1 and P1 , P2 AND P2 , and P3 and P3 is only 0.5 using both commands?

I understand that both command rely on different approaches. But not sure how to conclude whether or not to confirm relatedness. How do you think? If I trust the relatedness2, is 0.3 a confirmation for parent relationship?

Thanks thanks