Hello, For one of my exercises i have to create a pathway with the following requirements:
You will design a pathway suitable for E.coli. This pathway should at least posses the following characteristics:
- The activation molecule of the pathway should be IPTG
- If the pathway is NOT activated, the pathway should produce the protein GFP
- If the pathway IS activated, the GFP signal should turn OFF.
- If the pathway IS activated, it should produce a signal of your choice. This can be anything: protein production, cell death, a smell, whatever you can think of. But NOT GFP or ‘the absence of GFP’.
Hint: use the LUX operon in your design.
Could someone please help me create this pathway? I can't figure out how i create a suitable pathway for these requirements.
Thanks!
We won't do your homework for you. Tell us what you've tried and where you're stuck and someone will surely put you on the right track.
Is this a bioinformatics exercise or a molecular biology exercise?