Hello, For one of my exercises i have to create a pathway with the following requirements:
You will design a pathway suitable for E.coli. This pathway should at least posses the following characteristics:
- The activation molecule of the pathway should be IPTG
- If the pathway is NOT activated, the pathway should produce the protein GFP
- If the pathway IS activated, the GFP signal should turn OFF.
- If the pathway IS activated, it should produce a signal of your choice. This can be anything: protein production, cell death, a smell, whatever you can think of. But NOT GFP or ‘the absence of GFP’.
Hint: use the LUX operon in your design.
Could someone please help me create this pathway? I can't figure out how i create a suitable pathway for these requirements.