Creating a pathway with requirements
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4.3 years ago
JongD12 • 0

Hello, For one of my exercises i have to create a pathway with the following requirements:

You will design a pathway suitable for E.coli. This pathway should at least posses the following characteristics:

- The activation molecule of the pathway should be IPTG

- If the pathway is NOT activated, the pathway should produce the protein GFP

- If the pathway IS activated, the GFP signal should turn OFF.

- If the pathway IS activated, it should produce a signal of your choice. This can be anything: protein production, cell death, a smell, whatever you can think of. But NOT GFP or ‘the absence of GFP’.

Hint: use the LUX operon in your design.

Could someone please help me create this pathway? I can't figure out how i create a suitable pathway for these requirements.

Thanks!

Pathway Operon IPTG GFP • 752 views
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We won't do your homework for you. Tell us what you've tried and where you're stuck and someone will surely put you on the right track.

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Is this a bioinformatics exercise or a molecular biology exercise?

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