One of my colleagues is trying to do Sanger Sequencing. In her words:
"I am isolating DNA from tissues, amplifying the gene of interest, purifying it and sending it for sequencing. However, I get high-intensity peaks with lots of background: https://imgur.com/a/R1mwp
Do you have any suggestions I can try? I measure DNA conc. using Nanodrop before sending it to them, but it turns out higher than what I measured and gives high-intensity peaks. Although I see only one band corresponding to the desired product size. Can this be a primer issue? "
Can someone please suggest her steps to troubleshoot this.