Question: What'S The Difference Between 18S Rrna And 18S Rdna?
6
gravatar for Douglas S. Stones
7.2 years ago by
Melbourne, Australia
Douglas S. Stones260 wrote:

The 18S rRNA is frequently used in phylogeny (see http://en.wikipedia.org/wiki/18S_ribosomal_RNA). For example, GenBank ref. AY742884 (which I accessed from here) includes

LOCUS       AY742884                1846 bp    DNA     linear   INV 17-OCT-2005
DEFINITION  Physopelta gutta 18S ribosomal RNA gene, partial sequence.

which makes me confused, since it uses both terms DNA and RNA.

I'm after a straightforward explanation of the difference between 18S rRNA and 18S rDNA.

rrna rna dna • 14k views
ADD COMMENTlink modified 4.6 years ago by peacezah10 • written 7.2 years ago by Douglas S. Stones260

Thanks for taking the trouble answering this question.

ADD REPLYlink written 7.2 years ago by Douglas S. Stones260
11
gravatar for Dave Lunt
7.2 years ago by
Dave Lunt2.0k
Hull, UK
Dave Lunt2.0k wrote:

Although you sometimes see people getting muddled in Methods sections, nobody sequences rRNA in phylogenetic studies these days. They PCR from a DNA (not RNA) template and sequence that- it is DNA from start to finish. Not so at the start of the molecular phylogenetics era when rRNA sequences actually came directly from RNA extracted from cells. Giovanni is spot on though, and rRNA should be treated as a name for the product of the gene we are talking about, e.g. "I sequenced single stranded binding protein" usually doesn't mean you did anything with a protein, rather sequenced the gene and predicted the amino acids.

ADD COMMENTlink written 7.2 years ago by Dave Lunt2.0k
8
gravatar for Giovanni M Dall'Olio
7.2 years ago by
London, UK
Giovanni M Dall'Olio25k wrote:

"18S ribosomal RNA" is the name of the product of the gene. You should take it as a name, as if it were the name of a protein. The AY742884 entry identifies the DNA sequence that encodes for the 18S rRNA.

ADD COMMENTlink written 7.2 years ago by Giovanni M Dall'Olio25k
3
gravatar for Pierre Lindenbaum
7.2 years ago by
France/Nantes/Institut du Thorax - INSERM UMR1087
Pierre Lindenbaum103k wrote:

The rDNA is the transcribed DNA, giving a rRNA.

ADD COMMENTlink written 7.2 years ago by Pierre Lindenbaum103k
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