I'm processing NGS data and have a question.
I need to make my data have over 100,000,000 reads, so when my first processing is done, I check if they are good to go. When the bam files are not over 100,000,000 reads, I sequence those libraries which are more needed.
Here are the questions. 1. If I suppose my library, sample, sequencing machine and everything is the exactly the same, are the bam file which is merged after mapping and pre-merge fastq file, then mapped bam file same??
- And if they are same, can I merge bam files using samtools or sambamba??
Thank you very much.