Question: bacteriophage genome assembly
gravatar for najibveto
17 days ago by
Korea, Republic Of
najibveto30 wrote:

hello i am working on new bacteriophage i isolated from a bacteria. so i extracted the DNA from bacteriophage after isolation of the later using cesium gradient. i checked the DNA on electrophoresis and i get a size of genome around 17 kb. i send the dna to company and sequenced the genome. i got the results but most of blast gave bacterial proteins and one scaffold with a size of 20kb with no hits. so i want to confirm the results i got from company and i want to assemble the bacteriophage and possible annotation. what is the possible course of action i should do? and which software or pipeline i should use? thanks for your help.

ADD COMMENTlink modified 17 days ago by Buffo810 • written 17 days ago by najibveto30

What sequencing technology did you use? Illumina, PacBio or Oxford Nanopore?

ADD REPLYlink written 17 days ago by mschmid90
gravatar for Buffo
17 days ago by
Buffo810 wrote:

Before assembly confirm that you have sequenced a bacteriophage, map your reads to closest-reference, or to bacterial genomes.

ADD COMMENTlink written 17 days ago by Buffo810
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