Question: fastq quality scores
gravatar for nukkduko
16 months ago by
nukkduko0 wrote:

Hello everyone! I'm a little bit confused with fastq quality score. I've got fastq with reads like this:


What is phred quality score for first nucleotide? It's "a", but there is no small "a" in The Table: The Table

All that I know is that this score must be less or equal than 40.

fastq • 2.1k views
ADD COMMENTlink modified 16 months ago by Pierre Lindenbaum121k • written 16 months ago by nukkduko0
gravatar for cschu181
16 months ago by
cschu1811.7k wrote:

You're looking at a Phred33 table, but your read looks as if it might have a different encoding (Phred64):

ADD COMMENTlink written 16 months ago by cschu1811.7k

Yes, thanks for your help. With that table it works: Good for me table

ADD REPLYlink written 16 months ago by nukkduko0
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