Question: R fisher test two condition significantly different
gravatar for zqs1631
12 months ago by
zqs16310 wrote:

I have two condition data with two replicates.

condition1     condition2
rep1     1.729305     17.13960   
rep2     2.960495     18.59945

How can I test is the condition1 diff with condition2 significantly with fisher's test.

R fisher test • 366 views
ADD COMMENTlink modified 12 months ago by Michael Dondrup46k • written 12 months ago by zqs16310
gravatar for Michael Dondrup
12 months ago by
Bergen, Norway
Michael Dondrup46k wrote:

Hi zqs1631,

I don't see a direct connection to bioinformatics but, assuming you have a biological problem, anyhow the short answer is: You cannot. Fisher's exact test is for counts (discrete random variables), your data implies fractional values. Fisher's exact test does not apply here, you could try t-test instead. Note that for all reasonable biological experiments, 3 replicates is the absolute minimum, and often many more are required.

ADD COMMENTlink modified 12 months ago • written 12 months ago by Michael Dondrup46k

Hi, I agree with that answer and I just wanted to add that doing stats on 2 replicates makes no sense. Even if you get a significant result it doesn't mean anything in this case. Prefer to just make a graph and discuss the biological significance of this difference rather that the statistical difference.

ADD REPLYlink written 12 months ago by lagartija60
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