R fisher test two condition significantly different

Question

[zqs1631]

I have two condition data with two replicates.

condition1     condition2
rep1     1.729305     17.13960   
rep2     2.960495     18.59945

How can I test is the condition1 diff with condition2 significantly with fisher's test.

Answer 1

[Michael]

Hi zqs1631,

I don't see a direct connection to bioinformatics but, assuming you have a biological problem, anyhow the short answer is: You cannot. Fisher's exact test is for counts (discrete random variables), your data implies fractional values. Fisher's exact test does not apply here, you could try t-test instead. Note that for all reasonable biological experiments, 3 replicates is the absolute minimum, and often many more are required.

[lagartija]

Hi, I agree with that answer and I just wanted to add that doing stats on 2 replicates makes no sense. Even if you get a significant result it doesn't mean anything in this case. Prefer to just make a graph and discuss the biological significance of this difference rather that the statistical difference.