Library size factor

Question

[mhyunjunkang]

Hi all,

Can I get brief explanation why EdgeR and EBseq tools require library size factor for analysis?

Thanks, HJ

Answer 1

[h.mon]

Very brief: because of differences in sequencing depth between samples, which need to be take into account.

[mhyunjunkang]

Thank you for the hint. Does that mean that library size factor is the normalization factor?

[lakhujanivijay]

Thats one of the normalization factor