Tutorial: (Closed) raw count data- normalization
gravatar for ghmdsr
21 months ago by
ghmdsr30 wrote:

I got raw count data,which would consist of 32 samples and 20000 genes.

With this data, I have to normalize this.

first step; normalized gene expression values per sample.

second step: gene-wise normalization

Question1 : what is gene-wise normalization?

Question2 : how can i this two step?

Question3: in fist step , do i have to TMM normalization?

I'm beginner, but no one can help me in my workplace.. please help me.

rna-seq tutorial gene • 1.1k views
ADD COMMENTlink modified 21 months ago • written 21 months ago by ghmdsr30

Hello ghmdsr!

Questions similar to yours can already be found at:

We have closed your question to allow us to keep similar content in the same thread.

If you disagree with this please tell us why in a reply below. We'll be happy to talk about it.


ADD REPLYlink written 21 months ago by RamRS25k
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