I got raw count data,which would consist of 32 samples and 20000 genes.
With this data, I have to normalize this.
first step; normalized gene expression values per sample.
second step: gene-wise normalization
Question1 : what is gene-wise normalization?
Question2 : how can i this two step?
Question3: in fist step , do i have to TMM normalization?
I'm beginner, but no one can help me in my workplace.. please help me.