How to remove primers from a sequencing run with cutadapt
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5.9 years ago
Javad ▴ 150

Dear all,

I would like to use cutadapt to trim the primers from my sequencing run. I was just wondering when I give the sequence of my primer to cutadapt, do I also have to give the complementary sequence as well?

for example let's say my primer is: AATC

Then is this command "cutadapt -g AATC input.fa output.fa" sufficient to remove the primer sequence or I also have to run the following command:

cutadapt -g TTAG input.fa output.fa

Thanks in advance

RNA-Seq • 2.8k views
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from the manual:

Replace "ADAPTER" with the actual sequence of your 3' adapter. IUPAC wildcard characters are supported. The reverse complement is not automatically searched.

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